The Gadsden flag, proposing a rattlesnake and the phrase "Don't Tread on Me

https://archerdwgn450.yousher.com/the-legacy-of-civil-war-flags-symbolism-and-design-throughout-history

The Gadsden flag, presenting a rattlesnake and the phrase "Don't Tread on Me," turned into adopted via some Confederate instruments as a image of resistance against perceived oppression.